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. 1977 May;13(1-2):21-31.

[Histochemical and quantitative methods in the biopsy examination of skeletal muscles]

[Article in Czech]
Z Lukás, K Dvorák
  • PMID: 202410

[Histochemical and quantitative methods in the biopsy examination of skeletal muscles]

[Article in Czech]
Z Lukás et al. Cesk Patol. 1977 May.

Abstract

A complex bioptic examination of skeletal muscles as carried out in 280 excised samples has been subjected to a critical analysis. The examination suggested consists of histological and histochemical processing of native cryostat sections. Besides conventional staining methods the techniques recommended include the reaction for detection of myosin ATP-ase with and without acid preincubation, and reactions for NADH tertazolium reductase, acid phosphatase, nonspecific esterase, glycogen and lipids. Sections stained for ATP-ase are suitable for quantitative examinations such as assessing the diameters of muscle fibres.

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