Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1991 Mar 29;175(3):1131-8.
doi: 10.1016/0006-291x(91)91683-4.

Active recombinant C3a of human anaphylatoxin produced in Escherichia coli

Y Fukuoka  1 , A Yasui, T Tachibana
Affiliations

Active recombinant C3a of human anaphylatoxin produced in Escherichia coli

Y Fukuoka et al. Biochem Biophys Res Commun. .

Abstract

DNA sequence coding for the complete human C3a with 77 amino acids was divided into three portions, synthesized separately and constructed for expression in Escherichia coli. High expression of the recombinant C3a was achieved by an expression system using T7 polymerase. Purified recombinant C3a showed the same activities of ileum contraction and platelet aggregation of guinea pig as C3a purified from human serum.

PubMed Disclaimer

Publication types

MeSH terms

LinkOut - more resources