Complement-dependent phagocytosis of liposomes by macrophages: suppressive effects of "stealth" lipids

Abstract

We have previously reported that complement-opsonized liposomes composed of dimyristoyl phosphatidylcholine and cholesterol are actively phagocytozed by murine peritoneal macrophages and that such complement-induced phagocytosis can be suppressed by the presence of liposomal phosphatidylinositol (Proc. Natl. Acad. Sci. USA 81, 1984). We now report suppressive effects of other liposomal lipids, including monosialoganglioside (GM1) and sulfogalactosylceramide. Complement-dependent phagocytosis was almost completely suppressed by liposomes containing GM1 or phosphatidylinositol and partially suppressed when liposomes contained sulfogalactosylceramide. Although the mechanism of suppression of complement-induced phagocytosis by these liposomal lipids is not yet completely understood, it does not seem to involve the early stages of complement activation resulting in opsonization of liposomes with complement. We conclude that suppression of complement-induced phagocytosis by phosphatidylinositol, GM1, or sulfogalactosylceramide occurs at a step after liposome opsonization.