Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1978;9(1):56-9.
doi: 10.1159/000148921.

A simple method for concentration of enteroviruses and rotaviruses from cell culture harvests using membrane filters

S R Farrah, S M Goyal, C P Gerba, R H Conklin, C Wallis, J L Melnick, H L DuPont

A simple method for concentration of enteroviruses and rotaviruses from cell culture harvests using membrane filters

S R Farrah et al. Intervirology. 1978.

Abstract

Organic compounds in cell culture harvests known as membrane-coating components (MCC) prevent virus adsorption to membrane filters. Blending cell culture harvests with fluorocarbon removed the MCC and permitted adsorption of virus in acidified harvests to epoxy-fiberglass filters. Subsequent elution with high pH buffer resulted in recovery of greater than 90% of the virus with concentrations of up to 100-fold.

PubMed Disclaimer

MeSH terms

LinkOut - more resources