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. 1991 Apr 11;19(7):1413-9.
doi: 10.1093/nar/19.7.1413.

Hypersensitive site 4 of the human beta globin locus control region

S Pruzina  1 , O Hanscombe, D Whyatt, F Grosveld, S Philipsen
Affiliations
Free PMC article

Hypersensitive site 4 of the human beta globin locus control region

S Pruzina et al. Nucleic Acids Res. .
Free PMC article

Abstract

The Locus Control Region (LCR) of the human beta globin gene domain is defined by four erythroid-specific DNasel hypersensitive sites (HSS) located upstream of this multigene cluster. The LCR confers copy number dependent high levels of erythroid specific expression to a linked transgene, independent of the site of integration. To assess the role of the individual hypersensitive sites of the LCR, we have localized HSS4 to a 280bp fragment that is functional both in murine erythroleukaemia (MEL) cells and in transgenic mice. This fragment coincides with the major area of hypersensitivity 'in vivo' and contains a number of DNasel footprints. Bandshift analysis shows that these footprints correspond to binding sites for the erythroid specific proteins GATA1 and NF-E2 and a number of ubiquitous proteins, including jun/fos, Sp1 and TEF2.

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References

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