Intercalation of ethidium bromide into a triple-stranded oligonucleotide
- PMID: 2027760
- PMCID: PMC333910
- DOI: 10.1093/nar/19.7.1521
Intercalation of ethidium bromide into a triple-stranded oligonucleotide
Abstract
We have examined the ability of a cationic planar chromophore, ethidium bromide, to intercalate into a short, defined triple helix. Using UV absorption, fluorescence spectroscopy and a gel retardation assay we demonstrate that ethidium bromide is able to bind to a triple helix with a lower affinity than to the corresponding duplex. Energy transfer from base triplets to ethidium shows that ethidium is intercalated into the triple helix. The spectroscopic characteristics of ethidium intercalated into a triplex are similar to those observed for intercalation into duplex DNA.
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