The use of nuclease P1 in sequence analysis of end group labeled RNA
- PMID: 202925
- PMCID: PMC343228
- DOI: 10.1093/nar/4.12.4091
The use of nuclease P1 in sequence analysis of end group labeled RNA
Abstract
A method is described for the direct sequence analysis of 20-25 nucleotides from the termini of 5'- or 3'-end-group [32P] labeled RNA. The method involves partial endonucleolytic digestion of the labeled RNA with nuclease P1 (from Penicillium citrinum) followed by separation of the partial digestion products by two-dimensional homochromatography, the nucleotide sequence being determined by mobility shift analysis. This procedure has been applied to the sequence analysis of the terminal regions of tRNAs and of high molecular weight RNA, such as messenger RNA or viral RNA. A further application involves its use in conjunction with snake venom phosphodiesterase to determine the sequence of 5'-end group labeled oligonucleotides, containing modified bases, derived from T1 or pancreatic RNase digestion of tRNA.
Similar articles
-
Recent developments in methods for RNA sequencing using in vitro 32P-labeling.Fed Proc. 1980 Aug;39(10):2815-21. Fed Proc. 1980. PMID: 6157572
-
Use of in vitro 32P labeling in the sequence analysis of nonradioactive tRNAs.Methods Enzymol. 1979;59:58-109. doi: 10.1016/0076-6879(79)59072-7. Methods Enzymol. 1979. PMID: 220499 No abstract available.
-
Sequence analysis of T1 ribonuclease fragments of 18S ribosomal RNA by 5'-terminal labeling, partial digestion, and homochromatography fingerprinting.Nucleic Acids Res. 1977 Feb;4(2):339-52. doi: 10.1093/nar/4.2.339. Nucleic Acids Res. 1977. PMID: 190590 Free PMC article.
-
3H and 32P derivative methods for base composition and sequence analysis of RNA.Methods Enzymol. 1980;65(1):638-80. doi: 10.1016/s0076-6879(80)65065-4. Methods Enzymol. 1980. PMID: 6154873 No abstract available.
-
Studies on the sequence of the 3'-terminal region of turnip-yellow-mosaic-virus RNA.Eur J Biochem. 1977 Feb;72(3):465-78. doi: 10.1111/j.1432-1033.1977.tb11270.x. Eur J Biochem. 1977. PMID: 402264
Cited by
-
Synthesis of decadeoxyribonucleotides containing N6-methyladenine, N4-methylcytosine, and 5-methylcytosine: recognition and cleavage by restriction endonucleases (nucleosides and nucleotides part 74).Nucleic Acids Res. 1987 Jan 12;15(1):219-32. doi: 10.1093/nar/15.1.219. Nucleic Acids Res. 1987. PMID: 3029671 Free PMC article.
-
Minor-groove-modified oligonucleotides: synthesis of decadeoxynucleotides containing hypoxanthine, N2-methylguanine and 3-deazaadenine, and their interactions with restriction endonucleases Bgl II, Sau, 3AI, and Mbo I (Nucleosides and Nucleotides Part 75).Nucleic Acids Res. 1987 Apr 10;15(7):3059-72. doi: 10.1093/nar/15.7.3059. Nucleic Acids Res. 1987. PMID: 3031618 Free PMC article.
-
Identification and nucleotide sequence of the sup8-e UGA-suppressor leucine tRNA from Schizosaccharomyces pombe.Mol Gen Genet. 1979 May 4;172(2):221-8. doi: 10.1007/BF00268286. Mol Gen Genet. 1979. PMID: 289895
-
Nucleotide sequence at the 5' extremity of turnip yellow mosaic virus genome RNA.Proc Natl Acad Sci U S A. 1978 Jul;75(7):3168-72. doi: 10.1073/pnas.75.7.3168. Proc Natl Acad Sci U S A. 1978. PMID: 16592542 Free PMC article.
-
A viral ADP-ribosyltransferase attaches RNA chains to host proteins.Nature. 2023 Aug;620(7976):1054-1062. doi: 10.1038/s41586-023-06429-2. Epub 2023 Aug 16. Nature. 2023. PMID: 37587340 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
