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. 1991 Jan-Mar;35(1):221-3.

An improved method for the purification of blood cells of turkeys

J G Strain  1 , M Matsumoto
Affiliations
  • PMID: 2029254

An improved method for the purification of blood cells of turkeys

J G Strain et al. Avian Dis. 1991 Jan-Mar.

Abstract

Methods were developed to purify monocytes, heterophils, or erythrocytes from the whole blood of turkeys. After the bulk of thrombocytes was removed by centrifugation, blood was fractionated over a Ficoll-Hypaque discontinuous gradient. Five fractions were harvested separately, and two were further purified by attachment to a plastic surface and/or by lysis of erythrocytes. The monocyte fraction contained 41% +/- 1% monocytes, and the heterophil fraction contained 96% +/- 1% heterophils. The erythrocyte fraction showed a purity of 99.4% +/- 0.3%. These methods would be useful for various in vitro studies that require purified populations of blood cells.

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