doi: 10.1093/nar/4.12.4165.
End labeling of enzymatically decapped mRNA
- PMID: 202926
- PMCID: PMC343232
- DOI: 10.1093/nar/4.12.4165
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End labeling of enzymatically decapped mRNA
Nucleic Acids Res.
1977 Dec.
Free PMC article
Abstract
A method is presented for rapid and efficient 5' end labeling with 32P of capped mRNAs, by a series of three enzymatic reactions: the blocking nucleotide of the cap structure is removed by tobacco acid pyrophosphatase, and after dephosphorylation with alkaline phosphatase the 5' end is labeled with gamma-32-P-ATP and T4 polynucleotide kinase.
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