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. 1977 Dec;4(12):4165-74.
doi: 10.1093/nar/4.12.4165.

End labeling of enzymatically decapped mRNA

A Efstratiadis, J N Vournakis, H Donis-Keller, G Chaconas, D K Dougall, F C Kafatos
Free PMC article

End labeling of enzymatically decapped mRNA

A Efstratiadis et al. Nucleic Acids Res. 1977 Dec.
Free PMC article

Abstract

A method is presented for rapid and efficient 5' end labeling with 32P of capped mRNAs, by a series of three enzymatic reactions: the blocking nucleotide of the cap structure is removed by tobacco acid pyrophosphatase, and after dephosphorylation with alkaline phosphatase the 5' end is labeled with gamma-32-P-ATP and T4 polynucleotide kinase.

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References

    1. Biochemistry. 1976 May 18;15(10):2185-90 - PubMed
    1. Biochemistry. 1975 Mar 25;14(6):1225-9 - PubMed
    1. FEBS Lett. 1976 Jun 1;65(2):254-7 - PubMed
    1. FEBS Lett. 1976 Aug 15;67(2):209-13 - PubMed
    1. Cell. 1976 Dec;9(4 PT 2):747-60 - PubMed

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