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Comparative Study
. 1991 Jan;9(1):18-21.

[Molecular epidemiological markers of Legionella pneumophila in the Valencian Community (III). Peptide profiles]

[Article in Spanish]
C Muñoz  1 , V Catalán, R Borrás, J García-de-Lomas
Affiliations
  • PMID: 2029553
Comparative Study

[Molecular epidemiological markers of Legionella pneumophila in the Valencian Community (III). Peptide profiles]

[Article in Spanish]
C Muñoz et al. Enferm Infecc Microbiol Clin. 1991 Jan.

Abstract

Electrophoretic profiles using soluble peptides obtained from Legionella pneumophila serogroup 1 have been studied to be used as epidemiological markers. Using 8.5% polyacrylamide gels (SDS-PAGE) a unique profile common to environmental and clinical isolates has been detected showing 2 differential bands: a 150 kD and a 230 kD band present in only one clinical isolate. In order to achieve better resolution in the peptide profile 10 to 20% gradient SDS-PAGE has been used, confirming the existence of a common profile and 2 subtypes as well as another profile present in a smaller number of strains.

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