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. 2010 Apr 30;28(20):3582-7.
doi: 10.1016/j.vaccine.2010.02.088. Epub 2010 Mar 16.

Peak frequencies of circulating human influenza-specific antibody secreting cells correlate with serum antibody response after immunization

Jessica L Halliley  1 , Shuya Kyu, James J Kobie, Edward E Walsh, Ann R Falsey, Troy D Randall, John Treanor, Changyong Feng, Ignacio Sanz, F Eun-Hyung Lee
Affiliations

Peak frequencies of circulating human influenza-specific antibody secreting cells correlate with serum antibody response after immunization

Jessica L Halliley et al. Vaccine. .

Abstract

Upon vaccination, B cells differentiate into antibody secreting cells (ASCs) that migrate via the circulation to tissues. The kinetics of this response and the relationship of circulating ASCs to protective antibody titers have not been completely explored.

Methods: Influenza-specific and total-IgG ASCs were enumerated by Elispot and flow cytometry daily in the blood in 6 healthy adults after trivalent influenza vaccination (TIV).

Results: Peak H1-specific IgG ASC frequencies occurred variably from day 5 to 8 and correlated with the fold-rise rise in hemagglutination inhibition (HAI titers); r=0.91, p=0.006. H3-specific IgG ASC frequencies correlated less well, perhaps due to a mismatch of the H3 protein in the vaccine and that used in the Elispot assay. Peak frequencies of vaccine-specific and total-IgG ASCs were 0.3% and 0.8%, respectively, of peripheral blood mononuclear cells (PBMC). Peak TIV-, H1-, H3-, and total-IgG ASC frequencies were 1736+/-1133, 626+/-520, 592+/-463, and 4091+/-2019 spots/10(6) PBMC, respectively. Peak TIV-, H1-, and H3-specific IgG ASC of total-IgG ASC frequencies constituted 63%+/-21, 26%+/-10, 22%+/-17, respectively.

Conclusion: After immunization with inactivated influenza vaccine the peak in influenza-specific ASC frequencies is variable but correlates well with the magnitude of protective HAI responses.

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Figures

Figure 1
Figure 1
Kinetics of ASCs in the blood after immunization with trivalent influenza vaccine (TIV) in 6 subjects. ASC are identified that respond to all three hemagglutinin components (H1, H3 and B) of TIV. Total IgG ASCs are indicated by thin black triangles, BSA-specific ASC by open triangles, and TIV-specific ASC by bold black circles.
Figure 2
Figure 2
Kinetics of H1-specific ASC in the blood after TIV immunization in 6 subjects (solid triangles). Kinetics of H1-specific HAI antibody is shown by open triangles.
Figure 3
Figure 3
Kinetics of H3-specific ASC in the blood after TIV immunization in 6 subjects (solid circles). Kinetics of H3-specific HAI antibody is shown by open circles.
Figure 4
Figure 4
Comparison of circulating ASC or plasmablasts by flow cytometry and total IgG Elispot assays. (A) Plasmablasts identified by CD19+IgD−CD27hiCD38hi on day 0 and 6 for subject D. (B) Kinetics of ASC identified by flow cytometry and both total IgG and TIV-specific Elispots assays in subject D.
See this image and copyright information in PMC

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