Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2010:2010:757191.
doi: 10.1155/2010/757191. Epub 2010 Mar 14.

Protective effect and mechanisms of radix astragali injection on the intestinal mucosa of rats with obstructive jaundice

Affiliations

Protective effect and mechanisms of radix astragali injection on the intestinal mucosa of rats with obstructive jaundice

Zhang Xiping et al. Mediators Inflamm. 2010.

Abstract

Objective: To research the protective effects and mechanisms of Radix Astragali injection on the intestinal mucosa of rats with obstructive jaundice (OJ).

Methods: The rats were randomly divided into sham-operated, model control and Radix Astragali treated group. We observed the pathological changes of intestinal mucosa, expression levels of Bax and NF-kappaB proteins, and apoptosis indexes in intestinal mucosa as well as serum NO, MDA and SOD contents, respectively, on 7d, 14d, 21d and 28d after operation.

Results: The pathological severity score (on 7d and 14d), apoptotic indexes (on 14d) of the intestinal mucosa and serum MDA content (on 14d) of treated group were significantly lower than those in the model control group (P < .05). The serum SOD contents (on all time points) of treated group were significantly higher than those in the model control group (P < .05). The sham-operated group (on 21d) of the product of staining intensity and positive rate of Bax protein was significantly lower than model control group (P < .05).

Conclusion: Radix Astragali injection could protect the intestinal mucosa of OJ rats by increasing the content of SOD, reducing the content of MDA, inhibiting the apoptosis and relieving the pathological changes of intestinal mucosa.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Sham-operated group-28d mucous membrane of small intestine is approximately normal HE × 100.
Figure 2
Figure 2
Model control group-28d a large inflammatory cells infiltration in small intestinal wall HE × 200.
Figure 3
Figure 3
Model control group-28d a small quantity acidophile cells infiltration in intestinal lamina propria HE × 200.
Figure 4
Figure 4
Treated group-28d mucous membrane of small intestine is approximately normal HE × 200.
Figure 5
Figure 5
Model control group-28d mucous membrane of small intestine (+++) NF-κB × 200.
Figure 6
Figure 6
Model control group-28d mucous membrane of small intestine (++) NF-κB × 200.
Figure 7
Figure 7
sham-operated group-21d mucous membrane of small intestine (−) Bax × 200.
Figure 8
Figure 8
Model control group-21d mucous membrane of small intestine (+++) Bax × 200.
Figure 9
Figure 9
Model control group-21d mucous membrane of small intestine (++) Bax × 200.
Figure 10
Figure 10
Treated group-21d mucous membrane of small intestine (+) Bax × 200.
Figure 11
Figure 11
Treated group-21d a few apoptotic cells in mucous membrane of small intestine TUNEL × 200.

References

    1. Bansal V, Schuchert VD. Jaundice in the intensive care unit. Surgical Clinics of North America. 2006;86(6):1495–1502. - PubMed
    1. Beierle EA, Vauthey J-N, Moldawer LL, Copeland EM., III Hepatic tumor necrosis factor-α production and distant organ dysfunction in a murine model of obstructive jaundice. American Journal of Surgery. 1996;171(1):202–206. - PubMed
    1. Swank GM, Deitch EA. Role of the gut in multiple organ failure: bacterial translocation and permeability changes. World Journal of Surgery. 1996;20(4):411–417. - PubMed
    1. Assimakopoulos SF, Scopa CD, Vagianos CE. Pathophysiology of increased intestinal permeability in obstructive jaundice. World Journal of Gastroenterology. 2007;13(48):6458–6464. - PMC - PubMed
    1. Clements WDB, Erwin P, McCaigue MD, Halliday I, Barclay GR, Rowlands BJ. Conclusive evidence of endotoxaemia in biliary obstruction. Gut. 1998;42(2):293–299. - PMC - PubMed

Publication types

MeSH terms