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. 2010 Jul;167(1):31-6.
doi: 10.1016/j.jviromet.2010.03.006. Epub 2010 Mar 19.

Development of a rapid and comprehensive proteomics-based arboviruses detection system

Kenta Okamoto  1 , Yushirou Endo, Shingo Inoue, Takeshi Nabeshima, Phan Thi Nga, Posadas H Guillermo, Fuxun Yu, Do Phuong Loan, Bui Minh Trang, Filipinas F Natividad, Futoshi Hasebe, Kouichi Morita
Affiliations

Development of a rapid and comprehensive proteomics-based arboviruses detection system

Kenta Okamoto et al. J Virol Methods. 2010 Jul.

Abstract

A rapid and comprehensive protocol, which combines simple purification and liquid chromatography-electrospray ionisation-tandem mass spectrometry (LC-ESI/MS/MS), was developed for identification of arboviruses in infected culture fluid. Using this protocol, various arboviruses were detected including uncommon viruses that were described previously as Banna virus and Yunnan orbivirus. This approach is useful for the rapid screening of viral samples that cannot be identified by conventional gene amplification or immunological methods.

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