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. 2010 Apr;7(4):299-301.
doi: 10.1038/nmeth.1443. Epub 2010 Mar 21.

Direct determination of molecular haplotypes by chromosome microdissection

Li Ma  1 , Yan Xiao, Hui Huang, Qingwei Wang, Weinian Rao, Yue Feng, Kui Zhang, Qing Song
Affiliations

Direct determination of molecular haplotypes by chromosome microdissection

Li Ma et al. Nat Methods. 2010 Apr.

Abstract

Direct observation of haplotypes is still technically challenging. Here we report a method for the determination of haplotypes through chromosome microdissection. We determined human long-range chromosomal haplotypes with more than 98.85% accuracy at 24,245 genome-wide heterozygous single-nucleotide polymorphism (SNP) loci.

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Figures

Figure 1
Figure 1
The principle and results on chromosome 5 of the 7DDNA haplotyping method. (a) 7DDNA haplotyping procedure. (b) haplotyped chromosome 5, on which 2,514 SNPs were resolved. Each dot represents a SNP. Scale bars, 10 μM.
See this image and copyright information in PMC

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