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. 1977 Dec;28(4):428-30.

Isolation of living adult Onchocerca volvulus from nodules

H Schulz-Key, E J Albiez, D W Büttner
  • PMID: 203061

Isolation of living adult Onchocerca volvulus from nodules

H Schulz-Key et al. Tropenmed Parasitol. 1977 Dec.

Abstract

A technique for the isolation of adult Onchocerca volvulus from excised onchocercomata is described. The nodules are incubated in medium 199 containing 1-5 mg collagenase and 0.2 mg gentamicin per ml for 6-48 hours in a waterbath at 30-37 degrees C. A proportion of the worms can be isolated alive.

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