Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1991 Mar 25;19(6):1213-7.
doi: 10.1093/nar/19.6.1213.

CAAT/enhancer binding protein is able to bind to ATF/CRE elements

O Bakker  1 , M G Parker
Affiliations
Free PMC article

CAAT/enhancer binding protein is able to bind to ATF/CRE elements

O Bakker et al. Nucleic Acids Res. .
Free PMC article

Abstract

The transcription factor C/EBP is known to be able to bind to two different classes of sites, a CAAT-box and one present in a number of viral enhancers. In this paper we show using band-shift assays, methylation interference and footprinting that C/EBP is also able to bind with high affinity to ATF/CRE sites. Competition with mutant ATF sites and methylation interference indicate that C/EBP may be able to bind to the ATF/CRE sites by virtue of their homology to the enhancer core elements. Furthermore, we show that C/EBP is able to direct transcription from this site in transient transfection experiments and that mutations in the ATF binding site that impair DNA binding also effect the ability of C/EBP to stimulate transcription.

PubMed Disclaimer

References

    1. Genes Dev. 1987 Dec;1(10):1132-46 - PubMed
    1. Biochemistry. 1987 Nov 17;26(23):7234-8 - PubMed
    1. Genes Dev. 1988 Mar;2(3):267-81 - PubMed
    1. Cell. 1988 Jul 29;54(3):313-23 - PubMed
    1. Genes Dev. 1988 Sep;2(9):1089-100 - PubMed

MeSH terms