[Studies of the mucopolysaccharid metabolism in the juvenile angiofibroma (author's transl)]

Abstract

The mucopolysaccharide content of juvenile angiofibromas is investigated using histochemical and ultrahistochemical methods (histochemical in ten cases, ultrahistochemical in four cases). The investigations result in typical histochemical features and show the localization of acid mucopolysaccharides at electronmicroscopical level. The endothelium of the vessels exhibits a strong PAS-reaction and also shows acid groups at the cell surfaces. The interstitial tissue demonstrates neutral perjodatreactive substances as well as acid mucopolysaccharides. The latter substances mainly reacted in perifibrillar position. Electronmicroscopically the perjodreactive material was localized in the cytoplasma of typical fibroblasts and their modulations resembling histiocytes. The acid groups of carboxylated and sulfated mucopolysaccharides of the intercellular substance could be determined as hyaluronic acides and chondroitinsulfates (Chondroitin-4 and Chondroitin-6 sulfate) using the critical-electrolyte-concentration method after Scott and Dorling. Ultrastructurally these acid groups are localized as cell coat covering the fibroblasts and between the collagen fibrils. This histochemical investigation also supports the thesis of an organoid growth pattern of juvenile angiofibroma.