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. 2010 Mar;48(1):9-13.
doi: 10.3347/kjp.2010.48.1.9. Epub 2010 Mar 17.

PCR detection and molecular characterization of Pentatrichomonas hominis from feces of dogs with diarrhea in the Republic of Korea

Yun-Ah Kim  1 , Hye-Youn Kim, Shin-Hyeong Cho, Hyeong-Il Cheun, Jae-Ran Yu, Sang-Eun Lee
Affiliations

PCR detection and molecular characterization of Pentatrichomonas hominis from feces of dogs with diarrhea in the Republic of Korea

Yun-Ah Kim et al. Korean J Parasitol. 2010 Mar.

Abstract

Pentatrichomonas hominis is considered a commensal protozoan in the large intestine of a number of mammalian hosts, such as cats, dogs, and non-human primates. The resulting infections, which can induce diarrhea, have been attributed to opportunistic overgrowth of P. hominis. This study was performed to confirm the P. hominis infection and its molecular characterization from the feces of puppies with diarrhea. Fecal samples were obtained from 14 German shepherd puppies with diarrhea over 1 week (7 females and 7 males, 2-9 months of age) residing on a dog farm in August 2007. Species-specific PCR assay identified P. hominis 18S rRNA genes in 3 of the 14 puppies (1 female and 2 males; 1 aged 2 months and 2 aged 9 months). This phylogenetic analysis established that P. hominis belonged to the 1st clade, which is comprised of Bos taurus and Felines.

Keywords: 18S rRNA; Pentatrichomonas hominis; dog; phylogenetic analysis.

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Figures

Fig. 1
Fig. 1
P. hominis specific DNA was amplified by PCR in 3 of 14 German shepherd puppies with diarrhea. Lane M, 100 bp ladder; Lane P, positive control amplified at 339 bp; Lanes 2, 3, 4, 5, 6, 8, 9, 10 ,11, 12, and 14, negative; Lanes 1, 7, and 13, positive.
Fig. 2
Fig. 2
Comparison of alignment in 18S rRNA gene sequences of P. hominis amplified in the present study with others. (1) P. hominis in the present study; (2) P. hominis (GenBank accession number: AF124609); (3) P. hominis (ac: DQ412643); (4) P. hominis (ac: DQ412642); (5) P. hominis (ac: DQ412641); (6) P. hominis (ac: DQ412640); (7) P. hominis (ac: DQ899948). Dots indicate nucleotides identical to those of P. hominis and dashes are gaps inserted to optimize alignment. Numbering is based on the data registered in GenBank.
Fig. 3
Fig. 3
Neighbor-Joining (NJ) tree of Pentatrichomonas hominis from 18S rRNA gene sequences. P. hominis analyzed in this study are marked in rectangle. Data for tree construction obtained from GenBank Data Libraries of the NCBI. The length of each branch is proportional to the amount of evolutionary distance between the different species. Scale bar indicates 0.2 substitutions (corrected) per base pair.
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