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. 2010 Aug;30(6):869-76.
doi: 10.1007/s10571-010-9515-y. Epub 2010 Mar 25.

Screening genes related to development and injury of the mouse optic nerve by cDNA microarrays

Yunlai Liu  1 , Minghui Huang, Yanqi Zhang, Hongli Li, Lan Xiao, Jianjun Liu, Bibo Yuan, Maolin Qin, Chengren Li, Micheal Yang, Wenqin Cai
Affiliations

Screening genes related to development and injury of the mouse optic nerve by cDNA microarrays

Yunlai Liu et al. Cell Mol Neurobiol. 2010 Aug.

Abstract

The aim of this study was to screen genes related to the development and injury of the mouse optic nerve so as to provide possible target genes for gene-engineering therapy of central nervous system (CNS) injury. Gene expression was profiled by cDNA microarrays in the mouse superior colliculus at 8-time points during the development or following injury of the optic nerve; consequently, 1,095 highly expressed genes (ratio > or =2) were identified. Then, these genes were categorized functionally; there were 561 genes (51.19%) with unidentified functions and 534 genes (48.81%) with identified or partially identified functions. After discounting the overlapping genes, 486 genes with identified or partially identified functions were categorized into 17 functional groups. The 17 functional groups were as follows: I transcription regulation, II signal transduction, III protein synthesis, IV materials transporting, V RNA processing, VI metabolism-related genes, VII cell cycle or apoptosis-related genes, VIII extracellular matrix, IX protein folding and degradation, X cytoskeleton, XI histone metabolism, XII nervous system specific functional genes, XIII tumor related genes, XIV DNA replication and repair, XV axon growth and guidance, XVI immune response, and XVII cell adhesion. These genes may play key roles in the development, injury, and repairment of the optic nerve.

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Figures

Fig. 1
Fig. 1
Parts scanned images of cDNA microarrays. E18: embryonic 18 days, P7: postnatal 7 days, I7: injury 7 days, I21: injury 21 days. The mini figures in the right upper corners were overall views, and each figure was enlarged from the same location
Fig. 2
Fig. 2
The functional distribution of genes highly expressed only during optic-nerve development. The largest two functional groups are related to transcription regulation (15%) and signal transduction (15%)
Fig. 3
Fig. 3
The functional distribution of genes highly expressed only following injury. The largest two functional groups are related to materials transporting (18%) and metabolism (17%)
Fig. 4
Fig. 4
The functional distribution of genes highly expressed during both development and following injury. The largest functional group is related to protein synthesis (35%), and none related to cytoskeleton or cell adhesion is observed
See this image and copyright information in PMC

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