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Comparative Study
. 1991 May 15;88(10):4114-8.
doi: 10.1073/pnas.88.10.4114.

Primary structure of a cerulenin-binding beta-ketoacyl-[acyl carrier protein] synthase from barley chloroplasts

M Siggaard-Andersen  1 , S Kauppinen, P von Wettstein-Knowles
Affiliations
Comparative Study

Primary structure of a cerulenin-binding beta-ketoacyl-[acyl carrier protein] synthase from barley chloroplasts

M Siggaard-Andersen et al. Proc Natl Acad Sci U S A. .

Abstract

The radioactively labeled beta-ketoacyl thioester synthase inhibitor [3H] cerulenin was used to tag three dimeric barely chloroplast proteins (alpha alpha, alpha beta, and beta beta) from the stromal fraction. Oligonucleotides corresponding to amino acid sequences obtained from the purified proteins were used to generate with the polymerase chain reaction a probe for cDNAs encoding the beta subunit. cDNA sequencing revealed an open reading frame for 462 residues comprising the mature protein and a 35-amino acid transit peptide. The deduced amino acid sequence of the mature protein is homologous to the beta-ketoacyl-[acyl carrier protein] (ACP) synthase I [3-oxoacyl-ACP synthase; acyl-ACP:malonyl-ACP C-acyltransferase (decarboxylating), EC 2.3.1.41] of Escherichia coli. Under analogous experimental conditions [3H]cerulenin tagged a single dimeric protein from spinach chloroplasts.

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References

    1. Biochem Biophys Res Commun. 1972 Aug 7;48(3):649-56 - PubMed
    1. Carlsberg Res Commun. 1988;53(6):371-9 - PubMed
    1. Anal Biochem. 1988 Aug 15;173(1):201-5 - PubMed
    1. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 - PubMed
    1. Gene. 1983 Nov;25(2-3):263-9 - PubMed

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