Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2010 May;76(10):3383-6.
doi: 10.1128/AEM.01456-09. Epub 2010 Mar 26.

Risk of Listeria monocytogenes contamination of raw ready-to-eat seafood products available at retail outlets in Japan

Satoko Miya  1 , Hajime Takahashi, Tatsuya Ishikawa, Tateo Fujii, Bon Kimura
Affiliations

Risk of Listeria monocytogenes contamination of raw ready-to-eat seafood products available at retail outlets in Japan

Satoko Miya et al. Appl Environ Microbiol. 2010 May.

Abstract

Examination of Listeria monocytogenes prevalence among ready-to-eat foods in Japan revealed frequent (5.7 to 12.1%) contamination of minced tuna and fish roe products, and the isolates had the same virulence levels as clinical isolates in terms of invasion efficiency and infectivity in cell cultures and a murine infection model, respectively. Premature stop codons in inlA were infrequent (1 out of 39 isolates). Cell numbers of L. monocytogenes in minced tuna and salmon roe increased rapidly under inappropriate storage temperatures (from a most probable number [MPN] of 10(0) to 10(1)/g to an MPN of 10(3) to 10(4)/g over the course of 2 days at 10 degrees C). Thus, regulatory guidelines are needed for acceptable levels of L. monocytogenes in these foods.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
Virulence of L. monocytogenes isolates from RTE seafood in the mouse model. Seven-week-old female BALB/cCrSlc mice were infected via intravenous inoculation at 103 to 104 CFU. Bacteria were enumerated from the liver (black columns) and spleen (white columns) 3 days after infection. The rate of recovery was determined using the following formula: log (number of cells recovered)/log (number of cells inoculated) × 100. L. monocytogenes ATCC 51782, which has attenuated virulence due to the K220T substitution in PrfA (25), and L. innocua ATCC 33090 were used as negative controls.
See this image and copyright information in PMC

References

    1. Autio, T., S. Hielm, M. Miettinen, A.-M. Sjöberg, K. Aarnisalo, J. Björkroth, T. Mattila-Sandholm, and H. Korkeala. 1999. Sources of Listeria monocytogenes contamination in a cold-smoked rainbow trout processing plant detected by pulsed-field gel electrophoresis typing. Appl. Environ. Microbiol. 65:150-155. - PMC - PubMed
    1. Barbour, A. H., A. Rampling, and C. E. Hormaeche. 2001. Variation in the infectivity of Listeria monocytogenes isolates following intragastric inoculation of mice. Infect. Immun. 69:4657-4660. - PMC - PubMed
    1. Berrang, M. E., R. J. Meinersmann, J. K. Korthcutt, and D. P. Smith. 2002. Molecular characterization of Listeria monocytogenes isolated from a poultry further processing facility and from fully cooked product. J. Food Prot. 65:1574-1579. - PubMed
    1. Beuchat, L. R. 1996. Listeria monocytogenes incidence on vegetables. Food Control 7:223-228.
    1. Center for Food Safety and Applied Nutrition, Food and Drug Administration; Food Safety and Inspection Service, U.S. Department of Agriculture; and Centers for Disease Control and Prevention. 2003. Quantitative assessment of the relative risk to public health from foodborne Listeria monocytogenes among selected categories of ready-to-eat foods. http://www.fda.gov/Food/ScienceResearch/ResearchAreas/RiskAssessmentSafe....

Publication types

LinkOut - more resources