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. 2010 Jul;15(7):669-72.
doi: 10.1038/mp.2009.127. Epub 2010 Mar 30.

Insoluble DISC1-Boymaw fusion proteins generated by DISC1 translocation

X Zhou, Q Chen, K Schaukowitch, J R Kelsoe, M A Geyer

Insoluble DISC1-Boymaw fusion proteins generated by DISC1 translocation

X Zhou et al. Mol Psychiatry. 2010 Jul.
No abstract available

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Figures

Figure 1
Figure 1
Characterization of a novel type of Boymaw mRNA in human brain. (a) Identification of a novel exon E1b of Boymaw gene in human brain RNA by using 5′RACE. (b) E1b overlaps 198 basepairs with the 5′ UTR of CHORDC1 gene in a Cis-natural antisense orientation. (c) RT-PCR analysis of Boymaw transcripts in human thalamus and lymphoblastoid cells. (d). E1b is only present in multiple Boymaw isoforms in thalamus, not lymphoblastoid cells which express different Boymaw isoforms.
Figure 2
Figure 2
Insoluble DISC1-Boymaw proteins. (a) Construction of DISC1-Boymaw fusion (DB7) and Boymaw-DISC1 fusion (BD13) genes in pcDNA3.1-Zeocin vector. The expressed proteins form both cassettes were tagged with HA epitope. (b). Western blot analysis of the protein expression in HEK293 cells. The cells were harvested two days after transfection, and the proteins (supernatant fraction) were extracted in the same lysis buffer in the presence of 2% Sarkosyl (13). The pellet was collected and solubilized in SDS loading buffer. Equal amount of proteins from each sample were loaded for Western blot analysis. The control (C) was HEK293 cells transfected with pcDNA3.1-zeocin vector only. BD13 proteins were expressed abundantly in the supernatant fraction, and no detectable expression of BD7 was observed. In contrast, BD7 was detected only in the insoluble fraction (pellet). (c) Localization of BD13 and DB7 proteins in hippocampal neuronal cells. BD13 was expressed abundantly in the cytoplasm, and DB7 was much less expressed and formed “punctate” staining in cytoplasm and neuronal processes.
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References

    1. Blackwood DH, Fordyce A, Walker MT, St Clair DM, Porteous DJ, Muir WJ. Am J Hum Genet. 2001;69(2):428–433. - PMC - PubMed
    1. Millar JK, Wilson-Annan JC, Anderson S, Christie S, Taylor MS, Semple CA, et al. Hum Mol Genet. 2000;9(9):1415–1423. - PubMed
    1. Zhou X, Geyer MA, Kelsoe JR. Mol Psychiatry. 2008 Apr;13(4):361–363. - PMC - PubMed
    1. Gerstner JR, Landry CF. Neurochemical research. 2007 Feb;32(2):241–250. - PMC - PubMed
    1. Hahn JS. FEBS letters. 2005 Aug 15;579(20):4513–4519. - PubMed

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