Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2010 Feb 1;118(3):582-588.
doi: 10.1016/j.foodchem.2009.05.024.

Fractionation and evaluation of radical-scavenging peptides from in vitro digests of buckwheat protein

Yuanyuan Ma  1 , Youling L Xiong, Jianjun Zhai, Haining Zhu, Thomas Dziubla
Affiliations

Fractionation and evaluation of radical-scavenging peptides from in vitro digests of buckwheat protein

Yuanyuan Ma et al. Food Chem. .

Abstract

Buckwheat protein (BWP) isolate was subjected to a two-stage in vitro digestion (1 h pepsin followed by 2 h pancreatin at 37 °C). The antioxidant potential of the BWP digests was compared by assessing their capacity to scavenge 2,2'-azinobis (3-ethylbenzothiszoline-6-sulphonic acid) (ABTS(+•)) and hydroxyl ((•)OH) radicals. The 2-h pancreatin digest, which demonstrated the strongest activity against both radicals, was subjected to Sephadex G-25 gel filtration. Of the six fractions collected, fractions IV (456 Da) and VI (362 Da) showed the highest ABTS(+•) scavenging activity and were 23-27% superior to mixed BWP digest (P < 0.05). Fraction VI was most effective in neutralizing (•)OH and was 86 and 24% more efficient (P < 0.05) than mixed BWP digest and fraction IV, respectively. LC-MS/MS identified Trp-Pro-Leu, Val-Pro-Trp, and Val-Phe-Pro-Trp (IV), Pro-Trp (V) and tryptophan (VI) to be the prominant peptides/amino acid in these fractions.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Hydroxyl radical scavenging capacity of in vitro sequential digests of buckwheat protein. Means (n = 3) without a common letter differ significantly (P < 0.05). Sample solutions: 0.10 mg/mL protein.
Fig. 2
Fig. 2
ABTS+• scavenging capacity of in vitro sequential digests of buckwheat protein. Means (n = 3) without a common letter differ significantly (P < 0.05). Sample solutions: 0.159 mg/mL protein.
Fig. 3
Fig. 3
Sephadex G-25 gel filtration of the final in vitro digest (180 min total digestion time) of buckwheat protein (n = 23), and regression plot of log MW vs. elution volume (n = 3).
Fig. 4
Fig. 4
Hydroxyl radical scavenging capacity of gel filtration fractions of final in vitro digest (180 min total digestion time) of buckwheat protein. Means (n = 3) without a common letter differ significantly (P < 0.05). Sample solutions: 0.10 mg/mL protein.
Fig. 5
Fig. 5
ABTS+• scavenging capacity of gel filtration fractions of the final in vitro digest (180 min total digestion time) of buckwheat protein. Means (n = 3) without a common letter differ significantly (P < 0.05). Sample solutions: 0.159 mg/mL protein.
Fig. 6
Fig. 6
Extracted ion chromatograms (A, C, E, G, I) and tandem MS/MS spectra (B, D, F, H, J) of the prominent peptides present in gel filtration fractions IV (A–F), V (G; H), and VI (I; J). The peptides/amino acid were identified as Trp-Pro-Leu (B), Val-Pro-Trp (D), Val-Phe-Pro-Trp (F), Pro-Trp (H) and Trp (J).
See this image and copyright information in PMC

References

    1. Adler-Nissen J. Elsevier Applied Science. Vol. 427. New York, NY: 1986. Enzymic Hydrolysis of Food Proteins.
    1. Blau S, Rubinstein A, Bass P, Singaram C, Kohen R. Differences in the reducing power along the rat GI tract: Lower antioxidant capacity of the colon. Molecular and Cellular Biochemistry. 1999;194:185–191. - PubMed
    1. Boldyrev A, Johnson P. Carnosine and related compounds: antioxidant dipeptides. In: Johnson P, Boldyrev A, editors. Oxidative Stress at Molecular, Cellular and Organ Levels. Research Signpost; Kerala, India: 2002. pp. 101–114.
    1. Carrier J, Aghdassi E, Platt I, Cullen J, Allard JP. Effect of oral iron supplementation on oxidative stress and colonic inflammation in rats with induced coilitis. Alimentary Pharmacology and Therapeutics. 2001;15:1989–1999. - PubMed
    1. Chen HM, Muramoto K, Yamauchi F, Nokihara K. Antioxidant activity of designed peptides based on the antioxidative peptide isolated from digests of a soybean protein. Journal of Agricultural and Food Chemistry. 1996;44:2619–2623. - PubMed

LinkOut - more resources