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Comparative Study
. 2010 Jan;74(1):71-4.

Rapid serodiagnosis with the use of surface plasmon resonance imaging for the detection of antibodies against major surface protein A of Mycoplasma synoviae in chickens

Kiseok Oh  1 , Semi Lee, Jayoung Seo, Dongwoo Lee, Taejung Kim
Affiliations
Comparative Study

Rapid serodiagnosis with the use of surface plasmon resonance imaging for the detection of antibodies against major surface protein A of Mycoplasma synoviae in chickens

Kiseok Oh et al. Can J Vet Res. 2010 Jan.

Abstract
in English, French

Mycoplasma synoviae, a major worldwide pathogen in poultry, causes respiratory tract infection and arthritis in chickens and turkeys. Two major surface antigens of M. synoviae are encoded by a single gene, vlhA (variably expressed lipoprotein and hemagglutinin). The gene product is cleaved post-translationally to yield the lipoprotein major surface protein (MSP) B (MSPB) and the hemagglutinin MSPA. The availability of MSPA as an antigen for serodiagnosis was studied by means of a protein chip based on surface plasmon resonance imaging (SPRi). The diagnostic potential of SPRi for measurement of levels of antibody to MSPA was compared with that of a conventional enzyme-linked immunosorbent assay (ELISA) kit. The results from SPRi, a process that took only 1 h, were similar to those from ELISA. Therefore, MSPA can be used as an antigen for serologic studies, and SPRi, a label-free and high-throughput method, may be a valuable tool in avian serodiagnostic studies.

Mycoplasma synoviae est un agent pathogène de la volaille mondialement répandu, qui cause des infections du tractus respiratoire et de l’arthrite chez les poulets et les dindons. Deux antigènes de surface majeurs de M. synoviae sont codés par un gène unique, vlhA (lipoprotéine et hémagglutinine à expression variable). Le produit du gène est clivé post-traduction pour générer la lipoprotéine dénommée protéine majeure de surface (MSP) B (MSPB) et l’hémagglutinine MSPA. La disponibilité de MSPA comme antigène pour le sérodiagnostic a été étudiée grâce à une biopuce protéinique basée sur l’imagerie par résonance de plasmon de surface (SPRi). Le potentiel diagnostique de SPRi pour mesurer les niveaux d’anticorps contre MSPA a été comparé avec celui d’une trousse immuno-enzymatique (ELISA) conventionnelle. Les résultats de SPRi, un processus qui n’a pris que 1 h, étaient similaires à ceux de l’ELISA. Ainsi, MSPA peut être utilisé comme un antigène pour des études sérologiques, et SPRi, une méthode sans marqueur et à haut débit, comme un outil utile dans des études en sérodiagnostic aviaire.

(Traduit par Docteur Serge Messier)

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Figures

Figure 1
Figure 1
Top: Changes in signal intensity in surface plasmon resonance imaging (SPRi), representing the binding affinity between recombinant major surface protein A (rMSPA), immobilized on a gold chip by Prolinker B, and various dilutions of positive control serum (infected with Mycoplasma synoviae). Bottom: Original images of the signal intensity for each dilution.
Figure 2
Figure 2
Images from SPRi analysis of the antibodies against MSPA (rMSPA used as the antigen) in 302 field samples of chicken serum. N — negative controls; P — positive controls; MG — species controls (samples infected with M. gallisepticum).
See this image and copyright information in PMC

References

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