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. 2010 Feb 9;90(6):375-8.

[Combination of real-time polymerase chain reaction assay and serum galactomannan in the diagnosis of invasive aspergillosis in patients with hematological malignancies and recipients of hematopoietic stem cell transplantation]

[Article in Chinese]
Yu-qian Sun  1 , Yu Ji, Lan-ping Xu, Dai-hong Liu, Kai-yan Liu, Xiao-jun Huang
Affiliations
  • PMID: 20367932

[Combination of real-time polymerase chain reaction assay and serum galactomannan in the diagnosis of invasive aspergillosis in patients with hematological malignancies and recipients of hematopoietic stem cell transplantation]

[Article in Chinese]
Yu-qian Sun et al. Zhonghua Yi Xue Za Zhi. .

Abstract

Objective: To evaluate the role of real-time polymerase chain reaction (real-time PCR) assay combined with serum galactomannan (GM) detection in the diagnosis of invasive aspergillosis (IA) with hematological malignancies or hematopoietic stem cell transplantation.

Methods: Eighty patients were enrolled. PCR and GM were performed simultaneously on 205 serum samples. The agreement between these two assays and the diagnostic value of the combination were analyzed.

Results: There were 5 proven IA, 20 probable IA, 34 possible IA and 21 non-IA. Agreement between real-time PCR and GM was 77.5%. When both positive, the positive predictive value could reach 100%; when both negative, the negative predictive value could reach 90.9%.

Conclusion: PCR agrees well with GM. When these two assays are combined, invasive aspergillosis can be diagnosed more accurately.

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