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. 2010 Nov;61(5):451-7.
doi: 10.1007/s00284-010-9637-y. Epub 2010 Apr 6.

Characterization of Bombyx mori nucleopolyhedrovirus ORF109 that encodes a 25-kDa structural protein of the occlusion-derived virion

Yang Zhou  1 , Qin Yao, Hongxing Shen, Hengchuan Xia, Feng Lin, Keping Chen
Affiliations

Characterization of Bombyx mori nucleopolyhedrovirus ORF109 that encodes a 25-kDa structural protein of the occlusion-derived virion

Yang Zhou et al. Curr Microbiol. 2010 Nov.

Abstract

Bombyx mori nucleopolyhedrovirus (BmNPV) ORF109 (Bm109) is a gene present in many lepidopteran NPVs, but its function is unknown. In this study, Bm109 was characterized. The transcript of Bm109 was detected at 12-72 h postinfection (p.i.). Polyclonal antiserum raised to a His-BM109 fusion protein recognized BM109 in infected cell lysates from 24 to 72 h p.i., suggesting that Bm109 is a late gene. Localization of the BM109 in BV and ODV by western analyses demonstrated that BM109 was proteins of ODV.

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