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. 2010 Jun;76(11):3753-7.
doi: 10.1128/AEM.03080-09. Epub 2010 Apr 9.

Identification of diverse antimicrobial resistance determinants carried on bacterial, plasmid, or viral metagenomes from an activated sludge microbial assemblage

Larissa C Parsley  1 , Erin J Consuegra, Kavita S Kakirde, Andrew M Land, Willie F Harper Jr, Mark R Liles
Affiliations

Identification of diverse antimicrobial resistance determinants carried on bacterial, plasmid, or viral metagenomes from an activated sludge microbial assemblage

Larissa C Parsley et al. Appl Environ Microbiol. 2010 Jun.

Abstract

Using both sequence- and function-based metagenomic approaches, multiple antibiotic resistance determinants were identified within metagenomic libraries constructed from DNA extracted from bacterial chromosomes, plasmids, or viruses within an activated sludge microbial assemblage. Metagenomic clones and a plasmid that in Escherichia coli expressed resistance to chloramphenicol, ampicillin, or kanamycin were isolated, with many cloned DNA sequences lacking any significant homology to known antibiotic resistance determinants.

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Figures

FIG. 1.
FIG. 1.
HPLC profiles of clone A5 (A) and pSU2719 (B) supernatants from cultures grown overnight in the presence of chloramphenicol. (C) Negative control in which chloramphenicol was incubated overnight in cell-free medium. Filled arrows indicate the appearance of a by-product with a peak at 4.4 min, and the empty arrow indicates the native chloramphenicol peak. mAU, milli-absorbance units.
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