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. 2010 May;6(5):327-9.
doi: 10.1038/nchembio.350. Epub 2010 Apr 11.

A vancomycin photoprobe identifies the histidine kinase VanSsc as a vancomycin receptor

Kalinka Koteva  1 , Hee-Jeon Hong, Xiao Dong Wang, Ishac Nazi, Donald Hughes, Mike J Naldrett, Mark J Buttner, Gerard D Wright
Affiliations

A vancomycin photoprobe identifies the histidine kinase VanSsc as a vancomycin receptor

Kalinka Koteva et al. Nat Chem Biol. 2010 May.

Abstract

Inducible resistance to the glycopeptide antibiotic vancomycin requires expression of vanH, vanA and vanX, controlled by a two-component regulatory system consisting of a receptor histidine kinase, VanS, and a response regulator, VanR. The identity of the VanS receptor ligand has been debated. Using a synthesized vancomycin photoaffinity probe, we show that vancomycin directly binds Streptomyces coelicolor VanS (VanSsc) and this binding is correlated with resistance and required for vanH, vanA and vanX gene expression.

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Comment in

  • Antibiotics: vancomycin sensing.
    Arthur M. Arthur M. Nat Chem Biol. 2010 May;6(5):313-5. doi: 10.1038/nchembio.356. Nat Chem Biol. 2010. PMID: 20404817 No abstract available.

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