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. 1991 Jun;11(6):3365-8.
doi: 10.1128/mcb.11.6.3365-3368.1991.

Ca2+ is essential for multistep activation of the heat shock factor in permeabilized cells

B D Price  1 , S K Calderwood
Affiliations

Ca2+ is essential for multistep activation of the heat shock factor in permeabilized cells

B D Price et al. Mol Cell Biol. 1991 Jun.

Abstract

We have developed a novel permeabilized-cell system to study transcription mechanisms. In permeabilized cells, heat-induced activation of the heat shock factor and transcription of the hsp70 gene require Ca2+. Activation involves at least two steps: Ca(2+)- and heat-dependent activation of heat shock factor binding and a second step, prior to transcription of hsp70, that requires ATP and is sensitive to genistein, a protein kinase inhibitor.

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