Integrated DNA and fingerprint analyses in the identification of 60-year-old mummified human remains discovered in an Alaskan glacier
- PMID: 20384910
- DOI: 10.1111/j.1556-4029.2010.01356.x
Integrated DNA and fingerprint analyses in the identification of 60-year-old mummified human remains discovered in an Alaskan glacier
Abstract
This report describes the identification of a merchant mariner who perished in 1948 when Northwest Airlines Flight 4422, a DC-4 carrying 24 seamen and six crew members crashed into Mount Sanford, Alaska. Fifty-one years later, a human forearm and hand were found close by the wreckage of the plane, prompting identification efforts using DNA and fingerprints. There were significant challenges to both the fingerprint and DNA analyses. The hand was badly desiccated, making fingerprint friction-ridge detail almost invisible and the remains had been embalmed upon discovery, making DNA amplification difficult. We present the results of an interdisciplinary approach that successfully addressed these challenges and ultimately led to the identification of the remains. These efforts relied on efficient fingerprint rejuvenation and imaging techniques that improved print resolution, as well as new DNA extraction techniques optimized for aggressively embalmed remains.
Similar articles
-
Individual genetic identification of biological samples: a case of an aircraft accident.Forensic Sci Int. 2004 Dec 2;146 Suppl:S115-7. doi: 10.1016/j.forsciint.2004.09.035. Forensic Sci Int. 2004. PMID: 15639554
-
A novel approach for fingerprinting mummified hands.J Forensic Sci. 2008 Jul;53(4):952-5. doi: 10.1111/j.1556-4029.2008.00768.x. J Forensic Sci. 2008. PMID: 18489553
-
New optimized DNA extraction protocol for fingerprints deposited on a special self-adhesive security seal and other latent samples used for human identification.J Forensic Sci. 2011 Sep;56(5):1235-40. doi: 10.1111/j.1556-4029.2011.01853.x. Epub 2011 Aug 9. J Forensic Sci. 2011. PMID: 21827455
-
DNA identification in mass fatality incidents.Am J Forensic Med Pathol. 2011 Dec;32(4):393-7. doi: 10.1097/PAF.0b013e31822ed56e. Am J Forensic Med Pathol. 2011. PMID: 21897189 Review.
-
DNA fingerprinting using arbitrary primer technology (APT): a tool or a torment.Australas Biotechnol. 1994 Jul-Aug;4(4):232-6. Australas Biotechnol. 1994. PMID: 7765270 Review.
Cited by
-
Assessment of impact of DNA extraction methods on analysis of human remain samples on massively parallel sequencing success.Int J Legal Med. 2019 Jan;133(1):51-58. doi: 10.1007/s00414-018-1955-9. Epub 2018 Oct 19. Int J Legal Med. 2019. PMID: 30341454
-
A novel, 4-h DNA extraction method for STR typing of casework bone samples.Int J Legal Med. 2020 Mar;134(2):461-471. doi: 10.1007/s00414-019-02232-9. Epub 2020 Jan 2. Int J Legal Med. 2020. PMID: 31897668
-
Biological Sexing of a 4000-Year-Old Egyptian Mummy Head to Assess the Potential of Nuclear DNA Recovery from the Most Damaged and Limited Forensic Specimens.Genes (Basel). 2018 Mar 1;9(3):135. doi: 10.3390/genes9030135. Genes (Basel). 2018. PMID: 29494531 Free PMC article.
-
Mystery solved: the identification of the two missing Romanov children using DNA analysis.PLoS One. 2009;4(3):e4838. doi: 10.1371/journal.pone.0004838. Epub 2009 Mar 11. PLoS One. 2009. PMID: 19277206 Free PMC article.
-
RETRACTED: Genotyping strategies for tissues fixed with various embalming fluids for human identification, databasing, and traceability.J Forensic Sci. 2025 Jan;70(1):e1-e14. doi: 10.1111/1556-4029.15414. Epub 2023 Oct 31. J Forensic Sci. 2025. Retraction in: J Forensic Sci. 2025 Jan;70(1):410. doi: 10.1111/1556-4029.15684. PMID: 37904606 Free PMC article. Retracted.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources