[Application of immunochemical and chromatographic techniques for monitoring of trichothecene mycotoxins type A in grains]

Abstract

A sensitive HPLC-MS technique after immunoaffinity clean-up has been developed for the simultaneous determination of T-2 and HT-2 toxins of in the food grains. The limit of detection of method was 0,002 mg/kg for T-2 toxin and 0,005 mg/kg for HT-2 toxin. Comparison of existing immunochemical and chromatographic techniques of trichothecenes type A determination in grains was made. New combined scheme of monitoring of grain contamination by trichothecenes type A was proposed. It was based on screening by ELISAs followed by confirmation of positive samples by developed HPLC-MS technique with preliminary immune affinity purification of extracts. Proposed modification of immunochemical and chromatographic techniques allowed to lower the influence of grain matrix on the analytical results and to reduce of the probability obtaining of false-positive determination. Developed approach was used for determination of trichothecenes type A in natural contaminated grain samples.