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. 2008 Jan;70(1):96-9.
doi: 10.4103/0250-474X.40341.

HPLC Estimation of berberine in Tinospora cordifolia and Tinospora sinensis

G V Srinivasan  1 , K P Unnikrishnan, A B Rema Shree, Indira Balachandran
Affiliations

HPLC Estimation of berberine in Tinospora cordifolia and Tinospora sinensis

G V Srinivasan et al. Indian J Pharm Sci. 2008 Jan.

Abstract

A high-performance liquid chromatographic method for the estimation of berberine in the stem of Tinospora cordifolia (Willd.) Miers. ex Hook.f. and Thoms. and Tinospora sinensis (Lour.) Merrill is described. The dried stems of T. cordifolia and T. sinensis were defatted with petroleum ether (60-80 degrees ). The marc was dried and further extracted with methanol. The concentration of berberine in methanol extract was determined using a C-18 reverse phase column with a mobile phase of acetonitrile:water (10:90 v/v) at a flow rate of 0.6 ml/min and with UV detection at 266 nm. TLC and HPLC comparison of both the species revealed significant variation in the chemical constitution of the two species. This observation becomes important in the context of the use of T. sinensis in place of the genuine drug T. cordifolia.

Keywords: HPLC; Tinospora cordifolia; Tinospora sinensis; berberine.

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Figures

Fig. 1
Fig. 1
HPLC chromatogram of standard berberine. Berberine peak at the retetion time 8.6 min detected at a wavelength of 266 nm.
Fig. 2
Fig. 2
HPLC chromatogram of methanol extract of T. cordifolia. Peak at the retention time 8.6 min correspond to berberine.
Fig. 3
Fig. 3
HPLC chromatogram of methanol extract of T. sinensis. Peak at the retention time 8.6 min correspond to berberine.
See this image and copyright information in PMC

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