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. 1991 Mar;57(3):707-11.
doi: 10.1128/aem.57.3.707-711.1991.

Polymerase chain reaction identification of Vibrio vulnificus in artificially contaminated oysters

W E Hill  1 , S P Keasler, M W Trucksess, P Feng, C A Kaysner, K A Lampel
Affiliations

Polymerase chain reaction identification of Vibrio vulnificus in artificially contaminated oysters

W E Hill et al. Appl Environ Microbiol. 1991 Mar.

Abstract

DNAs extracted from Vibrio vulnificus seeded into oyster homogenates were evaluated as templates for the polymerase chain reaction. Several extraction procedures were examined, and it was determined that DNA recovered from cells lysed by guanidine isothiocyanate, extracted with chloroform, and precipitated with ethanol was most suitable for use as a polymerase chain reaction template. The region targeted was a 519-bp portion of the cytotoxin-hemolysin gene of V. vulnificus. This region was amplified only when DNA from this species was present in the homogenate. V. vulnificus seeded into oyster homogenates at an initial level of 10(2) CFU/g of oyster meat was consistently observed after 24 h of incubation in alkaline peptone water.

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References

    1. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 - PubMed
    1. Nucleic Acids Res. 1976 Sep;3(9):2303-8 - PubMed
    1. Biochemistry. 1979 Nov 27;18(24):5294-9 - PubMed
    1. Infect Immun. 1985 Apr;48(1):62-72 - PubMed
    1. Infect Immun. 1985 Dec;50(3):922-4 - PubMed