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Case Reports
. 2010 Jun;48(6):2250-2.
doi: 10.1128/JCM.01922-09. Epub 2010 Apr 14.

Detection of a mixed infection in a culture-negative brain abscess by broad-spectrum bacterial 16S rRNA gene PCR

Peter M Keller  1 , Silvana K Rampini, Guido V Bloemberg
Affiliations
Case Reports

Detection of a mixed infection in a culture-negative brain abscess by broad-spectrum bacterial 16S rRNA gene PCR

Peter M Keller et al. J Clin Microbiol. 2010 Jun.

Abstract

We describe the identification of two bacterial pathogens from a culture-negative brain abscess by the use of broad-spectrum 16S rRNA gene PCR. Simultaneous detection of Fusobacterium nucleatum and Porphyromonas endodontalis was possible due to a 24-bp length difference of their partially amplified 16S rRNA genes, which allowed separation by high-resolution polyacrylamide gel electrophoresis.

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Figures

FIG. 1.
FIG. 1.
Polyacrylamide gel electrophoresis of 16S rRNA gene PCR products. (A) Lane 1, molecular mass ladder XIV (Roche Diagnostics); lane 2, 16S rRNA gene amplicons obtained with patient sample I. (B) The setup for sample III is identical to that described for panel A. (C) Lane 1, amplicon of Porphyromonas endodontalis ATCC 35406T culture; lane 2, amplicon of Fusobacterium nucleatum subsp. nucleatum culture; lane 3, amplicons obtained with patient sample I; lane 4, amplicons obtained with patient sample III.
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References

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