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Comparative Study
. 2010 Jun;48(6):2282-4.
doi: 10.1128/JCM.00145-10. Epub 2010 Apr 14.

Indirect immunofluorescence assay for intra vitam diagnosis of avian bornavirus infection in psittacine birds

Sibylle Herzog  1 , Dirk Enderlein, Ursula Heffels-Redmann, Anne Piepenbring, Daniel Neumann, Erhard F Kaleta, Hermann Müller, Michael Lierz, Christiane Herden
Affiliations
Comparative Study

Indirect immunofluorescence assay for intra vitam diagnosis of avian bornavirus infection in psittacine birds

Sibylle Herzog et al. J Clin Microbiol. 2010 Jun.

Abstract

Different avian bornavirus (ABV) genotypes have recently been detected in psittacine birds with proventricular dilatation disease (PDD), an inflammatory fatal central and peripheral nervous system disorder. An indirect immunofluorescence assay (IIFA) for intra vitam demonstration of ABV-specific serum antibodies was established since reverse transcription-PCR (RT-PCR) assays may not detect all ABV variants.

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Figures

FIG. 1.
FIG. 1.
Indirect immunofluorescence assay for demonstration of ABV-specific antibodies, using BDV-infected MDCK cells. Note the brilliant granular fluorescence in the nucleus. Bar, 50 μm.
FIG. 2.
FIG. 2.
Indirect immunofluorescence assay for demonstration of ABV-specific antibodies, using ABV-infected CEC cells. Note the brilliant granular fluorescence in the nucleus. Bar, 100 μm; insert, 50 μm.
See this image and copyright information in PMC

References

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