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. 2010 Mar;26(1):108-11.
doi: 10.2987/09-5935.1.

A simple method for determining arbovirus transmission in mosquitoes

Sheri L Anderson  1 , Stephanie L Richards, Chelsea T Smartt
Affiliations

A simple method for determining arbovirus transmission in mosquitoes

Sheri L Anderson et al. J Am Mosq Control Assoc. 2010 Mar.

Abstract

We present a simplified method for the collection of mosquito saliva to determine Culex pipiens quinquefasciatus transmission of West Nile virus that can be used for experiments requiring large sample sizes.

Keywords: Vector competence; arbovirus; transmission rate.

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Figures

Fig. 1
Fig. 1
Collection of saliva from a mosquito into a capillary tube. (A) Close-up of a single Culex p. quinquefasciatus salivating into a capillary tube containing immersion oil. (B) Saliva from multiple mosquitoes can be collected at one time. (I) Mosquito affixed to sticky tape. (II) Plexiglass surface on top of white laboratory bench paper. (III) Capillary tube into which the proboscis is inserted and saliva is collected.
See this image and copyright information in PMC

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