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. 2010 Jul;54(7):3061-4.
doi: 10.1128/AAC.01834-09. Epub 2010 Apr 19.

Prevalence of fosfomycin resistance among CTX-M-producing Escherichia coli clinical isolates in Japan and identification of novel plasmid-mediated fosfomycin-modifying enzymes

Jun-ichi Wachino  1 , Kunikazu Yamane, Satowa Suzuki, Kouji Kimura, Yoshichika Arakawa
Affiliations

Prevalence of fosfomycin resistance among CTX-M-producing Escherichia coli clinical isolates in Japan and identification of novel plasmid-mediated fosfomycin-modifying enzymes

Jun-ichi Wachino et al. Antimicrob Agents Chemother. 2010 Jul.

Abstract

We evaluated the in vitro activity of fosfomycin against a total of 192 CTX-M beta-lactamase-producing Escherichia coli strains isolated in 70 Japanese clinical settings. Most of the isolates (96.4%) were found to be susceptible to fosfomycin. On the other hand, some of the resistant isolates were confirmed to harbor the novel transferable fosfomycin resistance determinants named FosA3 and FosC2, which efficaciously inactivate fosfomycin through glutathione S-transferase activity.

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Figures

FIG. 1.
FIG. 1.
Distribution of fosfomycin MICs for the 192 CTX-M-producing E. coli isolates.
FIG. 2.
FIG. 2.
Genetic environment of transferable fosfomycin resistance determinants and their neighboring regions in E. coli strains 08-624, 06-607, and C316.
FIG. 3.
FIG. 3.
Predicted amino acid sequences of fosfomycin resistance determinants. *, amino acid residues conserved among the seven fosfomycin resistance determinants; colons and dots, amino acid substitutions that result in homologous amino acid residues. Proteins (GenBank accession no.): FosATN (AAA98399); FosAPA (AAT49669); FosA2 (ACC85616); FosA3 (AB522970); ORF1 (AAP50248); FosC (AAZ14834); FosC2 (AB522969).
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References

    1. Beharry, Z., and T. Palzkill. 2005. Functional analysis of active site residues of the fosfomycin resistance enzyme FosA from Pseudomonas aeruginosa. J. Biol. Chem. 280:17786-17791. - PubMed
    1. Bernat, B. A., L. T. Laughlin, and R. N. Armstrong. 1999. Elucidation of a monovalent cation dependence and characterization of the divalent cation binding site of the fosfomycin resistance protein (FosA). Biochemistry 38:7462-7469. - PubMed
    1. Bernat, B. A., L. T. Laughlin, and R. N. Armstrong. 1997. Fosfomycin resistance protein (FosA) is a manganese metalloglutathione transferase related to glyoxalase I and the extradiol dioxygenases. Biochemistry 36:3050-3055. - PubMed
    1. Clinical and Laboratory Standards Institute. 2009. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved standard, 8th ed. Document M07-A8. Clinical and Laboratory Standards Institute, Wayne, PA.
    1. Endimiani, A., G. Patel, K. M. Hujer, M. Swaminathan, F. Perez, L. B. Rice, M. R. Jacobs, and R. A. Bonomo. 2009. In vitro activity of fosfomycin against blaKPC-containing Klebsiella pneumoniae isolates, including those nonsusceptible to tigecycline and/or colistin. Antimicrob. Agents Chemother. 54:526-529. - PMC - PubMed

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