doi: 10.1080/01971529108055060.
Rapid detection of antigen-specific mouse IgE by enzyme-linked immunosorbent assay (ELISA)
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- PMID: 2040707
- DOI: 10.1080/01971529108055060
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Rapid detection of antigen-specific mouse IgE by enzyme-linked immunosorbent assay (ELISA)
J Immunoassay.
1991.
Abstract
A rapid, sensitive, antigen-specific mouse IgE capture ELISA is described. A monoclonal rat anti-mouse IgE was used as the capture antibody, and a DNP-coupled BSA-biotinylated conjugate along with a peroxidase-avidin-biotin complex was utilized as the detection system. The lower detection limit of this assay is 8.5 ng/ml of antigen-specific IgE. With some modifications, this assay can be employed to screen for antigen specific antibodies of other isotypes and subtypes.
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