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Comparative Study
. 2010 Apr 21:8:32.
doi: 10.1186/1477-7819-8-32.

Genomic alterations of primary tumor and blood in invasive ductal carcinoma of breast

Ja Seong Bae  1 , Jin Soo Choi, Seung Ho Baik, Woo Chan Park, Byung Joo Song, Jeong Soo Kim, Young Lim, Sang Seol Jung
Affiliations
Comparative Study

Genomic alterations of primary tumor and blood in invasive ductal carcinoma of breast

Ja Seong Bae et al. World J Surg Oncol. .

Abstract

Background: Genomic alterations are important events in the origin and progression of various cancers, with DNA copy number changes associated with progression and treatment response in cancer. Array CGH is potentially useful in the identification of genomic alterations from primary tumor and blood in breast cancer patients. The aim of our study was to compare differences of DNA copy number changes in blood and tumor tissue in breast cancer.

Methods: DNA copy number changes in blood were compared to those in tumor tissue using array-comparative genomic hybridization in samples obtained from 30 breast cancer patients. The relative degree of chromosomal changes was analyzed using log2 ratios and data was validated by real-time polymerase chain reaction.

Results: Forty-six regions of gains present in more than 30% of the tissues and 70 regions of gains present in more than 30% of blood were identified. The most frequently gained region was chromosome 8q24. In total, agreement of DNA copy numbers between primary tumor and blood was minimal (Kappa = 0.138, p < 0.001).

Conclusion: Although there was only a slight agreement of DNA copy number alterations between the primary tumor and the blood samples, the blood cell copy number variation may have some clinical significance as compared to the primary tumor in IDC breast cancer patients.

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Figures

Figure 1
Figure 1
DNA copy number changes in a representative 30 IDC of each blood and tumor tissue. Results of array CGH analysis of 30 IDC human breast cancer tumor tissues and blood. The copy number fold change is shown on the y-axis and the genomic location is shown on the x-axis of panels A and B.
Figure 2
Figure 2
Comparison of array-CGH with Real-time PCR analysis. Each sample is depicted on the x-axis, fold change of array CGH is depicted by linear-ratios, and RT-PCR (y-axis) is delineated applying N-value. A threshold level >1 (linear-ration and N-value) indicates significant DNA copy number gain.
Figure 3
Figure 3
Frequency of gene copy number abnormalities in some chromosomal regions. Frequencies of significant genes increased or decreased in copy number at 30 IDC breast cancer samples.
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