Heart rate variability and muscle sympathetic nerve activity response to acute stress: the effect of breathing

Abstract

Previous research has suggested a relationship between low-frequency power of heart rate variability (HRV; LF in normalized units, LFnu) and muscle sympathetic nerve activity (MSNA). However, investigations have not systematically controlled for breathing, which can modulate both HRV and MSNA. Accordingly, the aims of this experiment were to investigate the possibility of parallel responses in MSNA and HRV (LFnu) to selected acute stressors and the effect of controlled breathing. After data were obtained at rest, 12 healthy males (28 +/- 5 yr) performed isometric handgrip exercise (30% maximal voluntary contraction) and the cold pressor test in random order, and were then exposed to hypoxia (inspired fraction of O(2) = 0.105) for 7 min, during randomly assigned spontaneous and controlled breathing conditions (20 breaths/min, constant tidal volume, isocapnic). MSNA was recorded from the peroneal nerve, whereas HRV was calculated from ECG. At rest, controlled breathing did not alter MSNA but decreased LFnu (P < 0.05 for all) relative to spontaneous breathing. MSNA increased in response to all stressors regardless of breathing. LFnu increased with exercise during both breathing conditions. During cold pressor, LFnu decreased when breathing was spontaneous, whereas in the controlled breathing condition, LFnu was unchanged from baseline. Hypoxia elicited increases in LFnu when breathing was controlled, but not during spontaneous breathing. The parallel changes observed during exercise and controlled breathing during hypoxia suggest that LFnu may be an indication of sympathetic outflow in select conditions. However, since MSNA and LFnu did not change in parallel with all stressors, a cautious approach to the use of LFnu as a marker of sympathetic activity is warranted.

Fig. 1

Individual and group (mean ± SD, n = 11) responses of low-frequency (LF in normalized units, LFnu) and muscle sympathetic nerve activity (MSNA; bursts/min) to spontaneous (SB) and controlled breathing (CB) conditions. Grouped response is indicated by the open circle and dashed line. ‡P < 0.01, significantly different from SB condition.

Fig. 2

Individual and group (mean ± SD, n = 8) responses of LFnu and MSNA to isometric handgrip exercise during SB and CB conditions. Grouped response is indicated by the open circle and dashed line. *P < 0.05, significantly different from baseline. †P < 0.05, significantly different from SB condition.

Fig. 3

Individual and group (mean ± SD, n = 10) responses of LFnu and MSNA to the cold pressor test during SB and CB conditions. Grouped response is indicated by the open circle and dashed line. *P < 0.05, significantly different from baseline. †P < 0.05, significantly different from SB condition.

Fig. 4

Individual and group (mean ± SD, n = 9) responses of LFnu and MSNA to hypoxia during SB and CB conditions. Grouped response is indicated by the open circle and dashed line. *P < 0.05, significantly different from baseline.