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. 2010 May 26;132(20):6914-6.
doi: 10.1021/ja101002f.

Identification of protein-bound dinitrosyl iron complexes by nuclear resonance vibrational spectroscopy

Zachary J Tonzetich  1 , Hongxin Wang, Devrani Mitra, Christine E Tinberg, Loi H Do, Francis E Jenney Jr, Michael W W Adams, Stephen P Cramer, Stephen J Lippard
Affiliations

Identification of protein-bound dinitrosyl iron complexes by nuclear resonance vibrational spectroscopy

Zachary J Tonzetich et al. J Am Chem Soc. .

Abstract

We have applied (57)Fe nuclear resonance vibrational spectroscopy (NRVS) to identify protein-bound dinitrosyl iron complexes. Intense NRVS peaks due to vibrations of the N-Fe-N unit can be observed between 500 and 700 cm(-1) and are diagnostic indicators of the type of iron dinitrosyl species present. NRVS spectra for four iron dinitrosyl model compounds are presented and used as benchmarks for the identification of species formed in the reaction of Pyrococcus furiosus ferredoxin D14C with nitric oxide.

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Figures

Chart 1
Chart 1
Examples of sulfur-ligated iron dinitrosyls.
Figure 1
Figure 1
57Fe partial vibrational density of states for (Et4N)[Fe(NO)2(SPh)2].
Figure 2
Figure 2
Comparison of the NRVS spectrum of RBS and the spectrum resulting from treatment of 57Fe-enriched P. furiosus Fd D14C with PAPA NONOate.
See this image and copyright information in PMC

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