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Review
. 2010 Apr 27;4(4):e615.
doi: 10.1371/journal.pntd.0000615.

Non-tuberculous mycobacteria in TB-endemic countries: are we neglecting the danger?

Krishnamoorthy Gopinath  1 , Sarman Singh
Affiliations
Review

Non-tuberculous mycobacteria in TB-endemic countries: are we neglecting the danger?

Krishnamoorthy Gopinath et al. PLoS Negl Trop Dis. .
No abstract available

PubMed Disclaimer

Conflict of interest statement

The authors have declared that no competing interests exist.

Figures

Figure 1
Figure 1. Genus- and species-specific diagnoses can be accomplished by using various PCR systems.
(A) Genus-specific hsp65 PCR. From lane M–14 are 100 bp marker, M tuberculosis, M. terrae, M. scrofulaceum, M. fortuitum, M. duvalii, M. smegmatis, M. chelonae, M. celatum, M. bovis (BCG), M. kansasii, M. bovis, M. flavescens, M. goodii, and M. avium, respectively. Note a 441 bp amplicon in all species. (B) TB complex-specific cfp32 PCR. From lane M–14 are 1,000 bp marker, M tuberculosis H37 Rv, M tuberculosis clinical isolates (3), M. bovis (BCG), M. bovis, M. scrofulaceum, M. fortuitum, M. duvalii, M. smegmatis, M. chelonae, M. celatum, M. kansasii, and M. flavescens, respectively. Note a 786 bp amplicon in M. tuberculosis (3) and M. bovis (2) species. (C) PCR amplification of 16–23 S-rRNA using genus-specific primers ITS-F and Mycom-2; Lane M, 100 bp Marker; Lanes 1, 4, 7, and 8 are M. tuberculosis isolates (121 bp); Lanes 2, 3, 5, and 6 are MAC isolates (144 bp); Lanes 9 and 12 are negative controls; and Lanes 10 and 11 are M. fortuitum isolates (223 bp). Other species of mycobacteria also yield amplicons of variable size specific for each species (unpublished data). (D) A multiplex PCR comprised of primers for Mycobacterium genus (targeting hsp65), M. tuberculosis (targeting cfp10), and M. avium complex (targeting MAC) specific genes. Lane M is 100 bp marker, Lanes 1 and 4 show M. tuberculosis single species infection (441 bp and 191 bp bands), Lanes 2 and 5 show M. avium complex single infection (441 bp and 144 bp bands), while Lanes 3 and 6 show mixed infection of M. tuberculosis + M. avium complex (3 bands of 441 bp, 191 bp, and 144 bp size). The last lane is negative control.
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