Application of the polymerase chain reaction (PCR) to detection of the aerolysin gene in whole cell cultures of beta-hemolytic Aeromonas hydrophila
- PMID: 2044689
Application of the polymerase chain reaction (PCR) to detection of the aerolysin gene in whole cell cultures of beta-hemolytic Aeromonas hydrophila
Abstract
Oligonucleotide primers were used in a polymerase chain reaction protocol to detect the aerolysin gene in Aeromonas hydrophila. Primers detected template DNA in hemolytic, cytotoxic and enterotoxic strains of A. hydrophila and no amplification was detected with hemolytic A. sobria, non-hemolytic A. hydrophila and A. caviae strains.
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