doi: 10.1016/0888-7543(91)90506-a.
Linear order of new and established DNA markers around the fragile site at Xq27.3
Affiliations
- PMID: 2045104
- DOI: 10.1016/0888-7543(91)90506-a
Item in Clipboard
Linear order of new and established DNA markers around the fragile site at Xq27.3
Genomics.
1991 May.
Abstract
We have used recombinant clones derived from microdissection of the fragile X region to characterize breakpoints around the fragile site at Xq27.3. So far, no microdissection markers derived from Xq28 material have been found, thus allowing a rapid screening for clones surrounding the fragile site by their presence in a somatic cell hybrid containing Xq27.2-Xqter. A total of 43 new DNA markers from Xq27 have been sublocalized within this chromosome band. Of these new DNA markers, 5 lie in an interval defined as containing the fragile X region. The saturation of Xq27 with DNA markers by microdissection demonstrates the power of this technique and provides the resources for generating a complete physical map of the region.
Similar articles
-
Microdissection of the fragile X region.Am J Hum Genet. 1990 Aug;47(2):181-6. Am J Hum Genet. 1990. PMID: 2378345 Free PMC article.
-
Physical map of human Xq27-qter: localizing the region of the fragile X mutation.Proc Natl Acad Sci U S A. 1991 Oct 1;88(19):8302-6. doi: 10.1073/pnas.88.19.8302. Proc Natl Acad Sci U S A. 1991. PMID: 1924290 Free PMC article.
-
Physical mapping studies on the human X chromosome in the region Xq27-Xqter.Genomics. 1987 Dec;1(4):297-306. doi: 10.1016/0888-7543(87)90028-0. Genomics. 1987. PMID: 3482420
-
DNA studies of X-linked mental retardation associated with a fragile site at Xq27.Am J Med Genet. 1986 Jan-Feb;23(1-2):633-42. doi: 10.1002/ajmg.1320230157. Am J Med Genet. 1986. PMID: 3513572 Review.
-
Fragile site Xq27.3 in a family without mental retardation.Clin Genet. 1992 Jan;41(1):33-5. doi: 10.1111/j.1399-0004.1992.tb03625.x. Clin Genet. 1992. PMID: 1633644 Review.
Cited by
-
Evaluation of human microdissection clones from the FRAXA region as tools for comparative mapping in the mouse: isolation of a conserved genomic clone close to FMR1.Mamm Genome. 1994 Sep;5(9):584-7. doi: 10.1007/BF00354936. Mamm Genome. 1994. PMID: 8000145 No abstract available.
-
Long-range walking techniques in positional cloning strategies.Mamm Genome. 1992;3(3):127-42. doi: 10.1007/BF00352457. Mamm Genome. 1992. PMID: 1617220 Review. No abstract available.
-
Molecular heterogeneity of the fragile X syndrome.Nucleic Acids Res. 1991 Aug 25;19(16):4355-9. doi: 10.1093/nar/19.16.4355. Nucleic Acids Res. 1991. PMID: 1886762 Free PMC article.
-
A YAC contig across the fragile X site defines the region of fragility.Nucleic Acids Res. 1991 Jun 25;19(12):3283-8. doi: 10.1093/nar/19.12.3283. Nucleic Acids Res. 1991. PMID: 2062644 Free PMC article.
-
Myotubular myopathy in a girl with a deletion at Xq27-q28 and unbalanced X inactivation assigns the MTM1 gene to a 600-kb region.Am J Hum Genet. 1995 May;56(5):1108-15. Am J Hum Genet. 1995. PMID: 7726166 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources
Medical