Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2010 Feb 26;15(2):66-9.
doi: 10.1186/2047-783x-15-2-66.

Evaluation of a new chemiluminescence immunoassay for diagnosis of syphilis

Xiaohui Mo  1 , Yuelan Jin, Yang Yang, Weizhong Hu, Weiming Gu
Affiliations

Evaluation of a new chemiluminescence immunoassay for diagnosis of syphilis

Xiaohui Mo et al. Eur J Med Res. .

Abstract

Objective: To assess the sensitivity, specificity, and feasibility of a new chemiluminescence immunoassay (CLIA) in the diagnosis of syphilis.

Methods: At first, a retrospective study was conducted, using 135 documented cases of syphilis and 30 potentially interfering samples and 80 normal sera. A prospective study was also performed by testing 2, 071 unselected samples for routine screening for syphilis. CLIA was compared with a nontreponemal test (TRUST) and a treponemal test (TPPA).

Results: There was an agreement of 100% between CLIA and TPPA in the respective study. The percentage of agreement among the 245 sera tested was 100.0%. Compared with TPPA, the specificity of CLIA was 99.9% (1817/1819), the sensitivity of CLIA was 100.0% (244/244) in the prospective study. CLIA showed 99.5% agreement with TPPA by testing 2, 071 unselected samples. And CLIA seemed to be more sensitive than TPPA in detecting the samples of primary syphilis.

Conclusions: CLIA is easy to perform and the indicator results are objective and unequivocal. It may be suitable for large-scale screening as a treponemal test substituted for TPPA.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Noh J, Ko HH, Yun Y. Evaluation of performance and false positivity of Mediace RPR test that uses a chemistry autoanalyzer. Korean J Lab Med. 2008;28(4):312–318. doi: 10.3343/kjlm.2008.28.4.312. - DOI - PubMed
    1. de Larrañaga G, Trombetta L, Wingeyer SP, Remondino G. False positive reactions in confirmatory tests for syphilis in presence of antiphospholipid antibodies: misdiagnosis with prognostic and social consequences. Dermatol Online J. 2006;12(4):22. - PubMed
    1. Körber A, Dissemond J, Lehnen MS. Syphilis with HIV coinfection. J Dtsch Dermatol Ges. 2004;2(10):833–840. doi: 10.1046/j.1439-0353.2004.04071.x. - DOI - PubMed
    1. Geusau A, Kittler H, Hein U. Biological false-positive tests comprise a high proportion of Venereal Disease Research Laboratory reactions in an analysis of 300,000 sera. Int J STD AIDS. 2005;16(11):722–726. doi: 10.1258/095646205774763207. - DOI - PubMed
    1. Marks R, Thomas-Kaskel AK, Schmidt D. Steroid refractory episcleritis as early manifestation of neurosyphilis. Eur J Med Res. 2006;11(7):309–312. - PubMed