Utility of a Luminex-based assay for multiplexed, rapid species identification of Candida isolates from an ongoing candidemia surveillance
- PMID: 20453902
- DOI: 10.1139/w10-003
Utility of a Luminex-based assay for multiplexed, rapid species identification of Candida isolates from an ongoing candidemia surveillance
Abstract
A Candida-specific Luminex-based assay with 11 probes was employed for multiplexed, rapid identification of 1182 Candida sp. isolates that were received as part of an ongoing population-based surveillance. All the Candida isolates were previously identified by a combination of methods, including phenotype and sequence analysis. Results showed that the Luminex assay was an attractive alternative to reference methods, as it is rapid, yields correct species identification, and is user friendly.
Similar articles
-
DNA probes for the rapid identification of medically important Candida species using a multianalyte profiling system.FEMS Immunol Med Microbiol. 2006 Mar;46(2):244-50. doi: 10.1111/j.1574-695X.2005.00031.x. FEMS Immunol Med Microbiol. 2006. PMID: 16487306
-
Real-time LightCycler polymerase chain reaction and melting temperature analysis for identification of clinically important Candida spp.J Microbiol Immunol Infect. 2009 Aug;42(4):290-5. J Microbiol Immunol Infect. 2009. PMID: 19949751
-
Infections due to Candida haemulonii: species identification, antifungal susceptibility and outcomes.Int J Antimicrob Agents. 2010 Jan;35(1):85-8. doi: 10.1016/j.ijantimicag.2009.08.009. Epub 2009 Sep 27. Int J Antimicrob Agents. 2010. PMID: 19786341
-
Multilocus sequence typing of pathogenic Candida species.Eukaryot Cell. 2008 Jul;7(7):1075-84. doi: 10.1128/EC.00062-08. Epub 2008 May 2. Eukaryot Cell. 2008. PMID: 18456859 Free PMC article. Review. No abstract available.
-
Candida kefyr as a cause of bloodstream infection and adjunctive role of biomarkers in its diagnosis.J Mycol Med. 2015 Mar;25(1):71-5. doi: 10.1016/j.mycmed.2014.04.002. Epub 2014 Oct 22. J Mycol Med. 2015. PMID: 25442911 Review.
Cited by
-
Simultaneous detection of seven enteric viruses associated with acute gastroenteritis by a multiplexed Luminex-based assay.J Clin Microbiol. 2012 Jul;50(7):2384-9. doi: 10.1128/JCM.06790-11. Epub 2012 Apr 18. J Clin Microbiol. 2012. PMID: 22518865 Free PMC article.
-
Emergence of azole-resistant Candida parapsilosis causing bloodstream infection: results from laboratory-based sentinel surveillance in South Africa.J Antimicrob Chemother. 2016 Jul;71(7):1994-2004. doi: 10.1093/jac/dkw091. Epub 2016 Apr 28. J Antimicrob Chemother. 2016. PMID: 27125552 Free PMC article.
-
Simultaneous Emergence of Multidrug-Resistant Candida auris on 3 Continents Confirmed by Whole-Genome Sequencing and Epidemiological Analyses.Clin Infect Dis. 2017 Jan 15;64(2):134-140. doi: 10.1093/cid/ciw691. Epub 2016 Oct 20. Clin Infect Dis. 2017. PMID: 27988485 Free PMC article.
-
Changes in incidence and antifungal drug resistance in candidemia: results from population-based laboratory surveillance in Atlanta and Baltimore, 2008-2011.Clin Infect Dis. 2012 Nov 15;55(10):1352-61. doi: 10.1093/cid/cis697. Epub 2012 Aug 14. Clin Infect Dis. 2012. PMID: 22893576 Free PMC article.
-
FKS mutations and elevated echinocandin MIC values among Candida glabrata isolates from U.S. population-based surveillance.Antimicrob Agents Chemother. 2010 Dec;54(12):5042-7. doi: 10.1128/AAC.00836-10. Epub 2010 Sep 13. Antimicrob Agents Chemother. 2010. PMID: 20837754 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical