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. 2010:2010:427694.
doi: 10.1155/2010/427694. Epub 2010 May 4.

Expression of monocarboxylate transporters 1, 2, and 4 in human tumours and their association with CD147 and CD44

Céline Pinheiro  1 , Rui M Reis, Sara Ricardo, Adhemar Longatto-Filho, Fernando Schmitt, Fátima Baltazar
Affiliations

Expression of monocarboxylate transporters 1, 2, and 4 in human tumours and their association with CD147 and CD44

Céline Pinheiro et al. J Biomed Biotechnol. 2010.

Abstract

Monocarboxylate transporters (MCTs) are important cellular pH regulators in cancer cells; however, the value of MCT expression in cancer is still poorly understood. In the present study, we analysed MCT1, MCT2, and MCT4 protein expression in breast, colon, lung, and ovary neoplasms, as well as CD147 and CD44. MCT expression frequency was high and heterogeneous among the different tumours. Comparing with normal tissues, there was an increase in MCT1 and MCT4 expressions in breast carcinoma and a decrease in MCT4 plasma membrane expression in lung cancer. There were associations between CD147 and MCT1 expressions in ovarian cancer as well as between CD147 and MCT4 in both breast and lung cancers. CD44 was only associated with MCT1 plasma membrane expression in lung cancer. An important number of MCT1 positive cases are negative for both chaperones, suggesting that MCT plasma membrane expression in tumours may depend on a yet nonidentified regulatory protein.

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Figures

Figure 1
Figure 1
Western-blot for CD44 (breast cancer cell line MDA MB 231) and CD147 (human colon tissue). The protein molecular weights observed are in accordance with the predicted for these proteins. FG: fully glycosylated, CG: core glycosylated.
Figure 2
Figure 2
Representative immunohistochemical expression of MCT1 in ovarian carcinoma (a), MCT2 in breast carcinoma (b), MCT4 in ovarian carcinoma (c), CD147 in ovarian carcinoma (d), MCT1 in lung cancer (e), and CD44 in lung cancer (f). Plasma membrane staining for both MCT1 (a) and CD147 (d) is shown in the same tumour area of an ovary cancer case and for both MCT1 (e) and CD44 (f) in the same area of lung cancer case.
See this image and copyright information in PMC

References

    1. Gatenby RA, Gillies RJ. Why do cancers have high aerobic glycolysis? Nature Reviews Cancer. 2004;4(11):891–899. - PubMed
    1. Warburg O. On the origin of cancer cells. Science. 1956;123(3191):309–314. - PubMed
    1. Izumi H, Torigoe T, Ishiguchi H, et al. Cellular pH regulators: potentially promising molecular targets for cancer chemotherapy. Cancer Treatment Reviews. 2003;29(6):541–549. - PubMed
    1. Fang JS, Gillies RD, Gatenby RA. Adaptation to hypoxia and acidosis in carcinogenesis and tumor progression. Seminars in Cancer Biology. 2008;18(5):330–337. - PMC - PubMed
    1. Wahl ML, Owen JA, Burd R, et al. Regulation of intracellular pH in human melanoma: potential therapeutic implications. Molecular Cancer Therapeutics. 2002;1(8):617–628. - PubMed

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