Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2010 May 15;9(10):2040-1.
doi: 10.4161/cc.9.10.11688. Epub 2010 May 15.

Contribution to neural and mesodermal lineages by porcine skin-derived progenitors (SKPs) in vivo

Ming-Tao Zhao, Martha W Bennett, Xia Zhang, Lee Spate, Kristin M Whitworth, Clifton N Murphy, August Rieke, Yong Zhang, Randall S Prather

Contribution to neural and mesodermal lineages by porcine skin-derived progenitors (SKPs) in vivo

Ming-Tao Zhao et al. Cell Cycle. .
No abstract available

PubMed Disclaimer

Figures

Figure 1
Figure 1
Contribution of porcine SKPs into neural and mesodermal lineages. (A) eGFP expression in injected day 5 IVF embryos 24 h post injection. (B) Positive control for anti-eGFP antibody: the section was from a uterus where placenta tissue was GFP positive (arrow) while epithelial endometrium was eGFP negative. This control fetus was created by nuclear transfer with eGFP transgenic donor cells. (C-H) Immunofluorescent staining of brain (C), kidney (E) and genital ridge (G) of chimeric fetuses. The corresponding negative controls with only secondary antibody were shown: brain (D), kidney (F) and genital ridge (H). The arrows show representative eGFP positive cell areas. (I) PCR results using genomic DNA from various tissues: brain (1-2), skin (3-4), kidney + genital ridge (5-6), liver (7-8) and body trunk (9-10). P: positive controls using the plasmid pEGFP-N1 as the template; N: negative control without template. W: wild-type porcine genomic DNA. GFP1 and GFP2 are different primer sets (Table 2). One fetus is in lanes 1, 3, 5, 7 & 9; while the other fetus is in lanes 2, 4, 6, 8 & 10. Scale bars: 100 μm (A); 50 μm (B-H).
See this image and copyright information in PMC

References

    1. Zhao M, et al. Cloning Stem Cells. 2009;11:111–22. - PMC - PubMed
    1. Toma JG, et al. Stem Cells. 2005;23:727–37. - PubMed
    1. Hunt DP, et al. Curr Opin Biotechnol. 2009;20:522–30. - PubMed
    1. Biernaskie J, et al. Cell Stem Cell. 2009;5:610–23. - PMC - PubMed
    1. Fernandes KJ, et al. Nat Cell Biol. 2004;6:1082–93. - PubMed

Publication types

LinkOut - more resources