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. 2010 Mar-Apr;15(2):020504.
doi: 10.1117/1.3366646.

Gold nanorods for fluorescence lifetime imaging in biology

Yinan Zhang  1 , Jun Yu, David J S Birch, Yu Chen
Affiliations
Free article

Gold nanorods for fluorescence lifetime imaging in biology

Yinan Zhang et al. J Biomed Opt. 2010 Mar-Apr.
Free article

Abstract

Two-photon luminescence (TPL) from gold nanorods shows considerable potential in biological imaging. We study the imaging of gold nanorods in Madin-Darby canine kidney (MDCK) cells using fluorescence lifetime imaging microscopy (FLIM). FLIM provides images with better contrast and sensitivity than intensity imaging. The characteristic fluorescence lifetime of gold nanorods is found to be less than 100 ps, which can be used to distinguish gold nanorods from other fluorescent labels and endogenous fluorophores in lifetime imaging.

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